Journal: Journal of Pest Science
Article Title: Sequencing of Tuta absoluta genome to develop SNP genotyping assays for species identification
doi: 10.1007/s10340-019-01116-6
Figure Lengend Snippet: Fig. 2 Flowchart describing the algorithm of the snp-id program. The search_iplex.py script of the snp-id program requires an input file (json) that specifies all the reference genomes (Fasta), replicate alignments (BAM), and multi-genome alignment (XMFA). It scans the multi-genome alignment for candidate SNPs and tests for (i) poly- morphisms in flanking regions, (ii) homozygosity, and (iii) evidence in other replicates. SNPs that satisfy all selection criteria are printed out in a format suitable for MassARRAY Typer 4.0 Assay Designer Software
Article Snippet: The list of SNPs that were identified using snp-id (Supplemental File 1) were then used as the input for the MassARRAY Typer 4.0 Assay Designer Software (Agena Bioscience, San Diego, CA, USA) to design iPLEX PCR and extension primers (Table 1).
Techniques: Selection, Software